ebm 2 growth media Search Results


serum free medium  (Thermo Fisher)
96
Thermo Fisher serum free medium
Serum Free Medium, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ebm+2+growth+media/Transferrin/pm12787141-56-20-36
Average 96 stars, based on 1 article reviews
serum free medium - by Bioz Stars, 2026-09
96/100 stars
  Buy from Supplier
vascular endothelial growth factor  (R&D Systems)
96
R&D Systems vascular endothelial growth factor
Fig. 3. Immunocytochemistry of pluripotency-associated markers in differentiated cells derived from piPSCs cultured in differentiation media. Immunostaining images of pluripotency-associated markers (OCT-3/4, SOX2 and NANOG) in porcine differentiated cells derived from piPSCs (OSKMNL) cultured in four differentiation media [StemDiff APEL TM 2 medium (APEL), StemDiff APEL TM 2 medium supple- mented with 50 ng/mL of Vascular <t>Endothelial</t> Growth Factor (APEL + <t>VEGF),</t> Endothelial Cell Basal Medium-2 (EBM-2) or Endothelial Cell Basal Medium-2 supplemented with 50 ng/mL of Vascular Endothelial Growth Factor (EBM-2 + VEGF)] on cultured plates coated with matrigel ® (1:40 dilution with DMEM/F-12 medium) for 8 days. Blue: Staining of Hoechst 33342, Red: Staining of OCT-3/4, SOX2 and NANOG protein. Merge: Hoechst 33342 signal and OCT-3/4, SOX2 and NANOG protein. Scale bar = 50 µm.
Vascular Endothelial Growth Factor, supplied by R&D Systems, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ebm+2+growth+media/Endothelial+Cell+Growth+Media+without+VEGF/10__12750_slash_jarb__38__3__109-37-58-65
Average 96 stars, based on 1 article reviews
vascular endothelial growth factor - by Bioz Stars, 2026-09
96/100 stars
  Buy from Supplier

Image Search Results


Fig. 3. Immunocytochemistry of pluripotency-associated markers in differentiated cells derived from piPSCs cultured in differentiation media. Immunostaining images of pluripotency-associated markers (OCT-3/4, SOX2 and NANOG) in porcine differentiated cells derived from piPSCs (OSKMNL) cultured in four differentiation media [StemDiff APEL TM 2 medium (APEL), StemDiff APEL TM 2 medium supple- mented with 50 ng/mL of Vascular Endothelial Growth Factor (APEL + VEGF), Endothelial Cell Basal Medium-2 (EBM-2) or Endothelial Cell Basal Medium-2 supplemented with 50 ng/mL of Vascular Endothelial Growth Factor (EBM-2 + VEGF)] on cultured plates coated with matrigel ® (1:40 dilution with DMEM/F-12 medium) for 8 days. Blue: Staining of Hoechst 33342, Red: Staining of OCT-3/4, SOX2 and NANOG protein. Merge: Hoechst 33342 signal and OCT-3/4, SOX2 and NANOG protein. Scale bar = 50 µm.

Journal: Journal of Animal Reproduction and Biotechnology

Article Title: Limited in vitro differentiation of porcine induced pluripotent stem cells into endothelial cells

doi: 10.12750/jarb.38.3.109

Figure Lengend Snippet: Fig. 3. Immunocytochemistry of pluripotency-associated markers in differentiated cells derived from piPSCs cultured in differentiation media. Immunostaining images of pluripotency-associated markers (OCT-3/4, SOX2 and NANOG) in porcine differentiated cells derived from piPSCs (OSKMNL) cultured in four differentiation media [StemDiff APEL TM 2 medium (APEL), StemDiff APEL TM 2 medium supple- mented with 50 ng/mL of Vascular Endothelial Growth Factor (APEL + VEGF), Endothelial Cell Basal Medium-2 (EBM-2) or Endothelial Cell Basal Medium-2 supplemented with 50 ng/mL of Vascular Endothelial Growth Factor (EBM-2 + VEGF)] on cultured plates coated with matrigel ® (1:40 dilution with DMEM/F-12 medium) for 8 days. Blue: Staining of Hoechst 33342, Red: Staining of OCT-3/4, SOX2 and NANOG protein. Merge: Hoechst 33342 signal and OCT-3/4, SOX2 and NANOG protein. Scale bar = 50 µm.

Article Snippet: In vitro differentiation of piPSCs into endothelial cells For in vitro differentiation of piPSCs into endothelial cells, piPSCs (OSKMNL) were passaged by manually picking the colonies and then seeded onto 0.5% gelatin-coated culture plates in four differentiation media [Endothelial Cell Basal Medium 2 (EBM-2; Lonza, Muenchensteinerstrasse, Basel, Switzerland), Endothelial Cell Basal Medium 2 supplemented with 50 ng/mL of Vascular Endothelial Growth Factor (EBM-2 + VEGF; R&D Systems, Minneapolis, MN, USA), StemDiff APELTM 2 medium (APEL-2; STEMCELL Technologies, Vancouver, BC, Canada), StemDiff APELTM 2 medium supplemented with 50 ng/mL of Vascular Endo- thelial Growth Factor (APEL-2 + VEGF)] on cultured plates coated with matrigel® (CORNING, NY, USA) (1:40 dilution with DMEM/F-12 medium) for 8 days at 39°C.

Techniques: Immunocytochemistry, Derivative Assay, Cell Culture, Immunostaining, Staining

Fig. 5. Flow cytometry of endothelial cell marker (CD-31) in differentiated cells derived from piPSCs cultured in differentiation media. Flow cytometry of endothelial cell marker (CD-31) expression in porcine differentiated cells derived from piPSCs (OSKMNL) cultured in four differentiation media [StemDiff APEL TM 2 medium (APEL), StemDiff APEL TM 2 medium supplemented with 50 ng/mL of Vascular En- dothelial Growth Factor (APEL + VEGF), Endothelial Cell Basal Medium-2 (EBM-2) and Endothelial Cell Basal Medium-2 supplemented with 50 ng/mL of Vascular Endothelial Growth Factor (EBM-2 + VEGF)] on cultured plates coated with matrigel ® (1:40 dilution with DMEM/F-12 medium) for 8 days was analyzed. Swine umbilical cord vein endothelial cells (SUVEC) were used as the positive control. Cells in blue were treated with PE-conjugated CD-31 and cells in red were not treated with PE-conjugated CD-31.

Journal: Journal of Animal Reproduction and Biotechnology

Article Title: Limited in vitro differentiation of porcine induced pluripotent stem cells into endothelial cells

doi: 10.12750/jarb.38.3.109

Figure Lengend Snippet: Fig. 5. Flow cytometry of endothelial cell marker (CD-31) in differentiated cells derived from piPSCs cultured in differentiation media. Flow cytometry of endothelial cell marker (CD-31) expression in porcine differentiated cells derived from piPSCs (OSKMNL) cultured in four differentiation media [StemDiff APEL TM 2 medium (APEL), StemDiff APEL TM 2 medium supplemented with 50 ng/mL of Vascular En- dothelial Growth Factor (APEL + VEGF), Endothelial Cell Basal Medium-2 (EBM-2) and Endothelial Cell Basal Medium-2 supplemented with 50 ng/mL of Vascular Endothelial Growth Factor (EBM-2 + VEGF)] on cultured plates coated with matrigel ® (1:40 dilution with DMEM/F-12 medium) for 8 days was analyzed. Swine umbilical cord vein endothelial cells (SUVEC) were used as the positive control. Cells in blue were treated with PE-conjugated CD-31 and cells in red were not treated with PE-conjugated CD-31.

Article Snippet: In vitro differentiation of piPSCs into endothelial cells For in vitro differentiation of piPSCs into endothelial cells, piPSCs (OSKMNL) were passaged by manually picking the colonies and then seeded onto 0.5% gelatin-coated culture plates in four differentiation media [Endothelial Cell Basal Medium 2 (EBM-2; Lonza, Muenchensteinerstrasse, Basel, Switzerland), Endothelial Cell Basal Medium 2 supplemented with 50 ng/mL of Vascular Endothelial Growth Factor (EBM-2 + VEGF; R&D Systems, Minneapolis, MN, USA), StemDiff APELTM 2 medium (APEL-2; STEMCELL Technologies, Vancouver, BC, Canada), StemDiff APELTM 2 medium supplemented with 50 ng/mL of Vascular Endo- thelial Growth Factor (APEL-2 + VEGF)] on cultured plates coated with matrigel® (CORNING, NY, USA) (1:40 dilution with DMEM/F-12 medium) for 8 days at 39°C.

Techniques: Flow Cytometry, Marker, Derivative Assay, Cell Culture, Expressing, Positive Control